Department of Hematology

Patient Care

OPD Schedule

S.No.	Name of the Clinic	Days on which held
1.	Hematology OPD (New Registrations and Follow up)	Tuesday and Thursday
2.	Chemotherapy OPD	Monday to Saturday
3.	MPN Clinic	Wednesday
4.	Myeloma Clinic	Friday
5.	Aplastic Anemia Clinic	Monday
6.	Bone marrow transplant OPD	Tuesday and Thursday

• The Dept has a General Haematology Ward, an acute leukemia ward, a BMT ward, a chemotherapy ward and a day care facility.
• The Dept of Haematology is the only centre of the State of Uttar Pradesh, performing Haemopoietic Stem cell/Bone Marrow Transplants (HSCT/BMT). Till date 94 transplants have been performed, 63 allogeneic and 30 autologous, with a 100-day mortality of 19% and overall survival of 65.2%. The Dept has also performed successfully two haploidentical HSCT.
• The Dept also has a dedicated Acute Myeloid Leukaemia (AML) ward with 06 HEPA filter isolation rooms. Strict barrier nursing care and ready availability of intensive care facilities in this ward has resulted in less than 5% mortality in AML inductions.
• The Dept also offers round the clock services for granulocyte transfusions which has dramatically brought down the mortality in patients with neutropenic sepsis, especially in those with multi drug resistant organisms.
• For highly specialised care and data management, the Dept runs Specialty OPD Clinics from Monday-Saturday, with each day of the week earmarked for a set of haematological disorders.
• The Dept. has a nodal role to play in the haemophilia care of the State of U.P. and coordinates with 26 district hemophilia treatment centres of the State, to provide comprehensive Haemophilia care including Clotting Factor Concentrate (CFC) replacement therapy, Immune tolerance induction therapy, physiotherapy and genetic counselling. The National Health Mission (NHM)/UP State Govt provides funds for supply of free CFC to all the haemophilia patients of the State. Dr Soniya Nityanand has been designated as the State Nodal Officer (Uttar Pradesh) for the Hemophilia Program.
• The Dept gets financial support for HSCT/BMT of thalassemia patients, under an MOU signed between the Coal India Limited (CIL)-Ministry of Health & Family Welfare (MoH&FW) and SGPGIMS, in 2018. In addition, the Department also offers services for haemoglobinopathy diagnosis, genetic counselling of families and State Govt supported free transfusion support and chelation for thalassemic children.
• The Dept through the Cuddles Foundation, is one of the few centres in the country offering nutritional support to its Paediatric patients, which plays a vital role in the outcome to chemotherapy in paediatric patients

Inpatient Services

In patient ward beds: 63

Chemotherapy Ward: 14

Acute Leukemia Ward: 25

General Ward: 24

Day Care Chemotherapy beds: 10

Bone Marrow Transplant beds: 09

Laboratories Pertaining to the Department

 

1.Morphology lab. The lab deals with complete blood count, peripherals smears examination, bone marrow aspiration smears, bone marrow biopsy, Lymph node biopsy and immunohistochemistry services. The lab is equipped with a decahead and trihead microscope used reporting and teaching. Morphology lab is enrolled in external quality assurance (EQA) program conducted by AIIMS, New Delhi. List of Services and turnaround time (TAT) are:

Services	TAT	Services	TAT
CBC	6 hrs	Serum Erythropoietin	7 days
MCV/MCH/MCHC	6 hrs	Specific Immunophenotyping	4-5 days
Mean Platelet Volume	6 hrs	Lymphoma Screening panel	4-5 days
Red Cell distribution width	6 hrs	Lymphoma Diagnostic Panel	4-5 days
Peripheral Smear Examination	24 hrs	Leukaemia Panel	4-5 days
Bone Marrow  Aspirate	48 hrs	Review of BM aspirate/Imprint	1-2 days
Bone Marrow-Biopsy	4 days	Small Biopsy/Lymph Node Biopsy	3-4   days

 

2.     Coagulation Lab: The coagulation lab of the department is one of the state-of-the-art labs which perform several routine as well as specialized investigations related to bleeding and coagulation disorders. The lab is enrolled in external quality assurance (EQA) program conducted by CMC Vellore. The lab is also one of the nodal centres for regional training programme conducted by world federation of haemophilia (WFH).List of Services and turnaround time (TAT) are as follows:

Services	TAT	Services	TAT
Caogulation Screen (PT, aPTT, TT)	4hrs	Protein C	1week
Factor VIII/FIX  Levels	3 Day	Protein S	1week
Factor VIII inhibitor assay	3 Day	Anti B2 GP1	1week
Factor IX inhibitor assay	2Day	Antithrombin III	1week
Factor XIII Screening assay	1Day	Lupus Anticoagulant	4Days
Factor X functional Assay	1Day	Factor V Leiden mutation	4Days
Factor V Functional Assay	1Day	Platelet Function test	8hrs
Factor VII functional Assay	1Day	Ristocetin Induced Platelet aggregation	8hrs
Von Willebrand Assay	1Day	Fibrinogen Assay	4 hrs

 

3.     Flow Cytometry Lab: The lab is involved on a date to day basis in the diagnosis, classification, and monitoring of hematological disorders. It is one of the few labs in India which perform is 10 colour experiments on a routine basis. The lab is enrolled in the external quality assurance/Inter lab comparison program (ILCP) conducted by Tata Memorial Hospital, Mumbai. List of Services and turnaround time (TAT) are as follows:

Services	TAT	Services	TAT
Acute Leukemia Screen Panel	2 days	CLL panel	2 days
Acute Leukemia Panel -Comprehensive	2-3 days	CLPD panel	2-3 days
PNH Screening Panel	2 days	Osmotic Fragility Test by Flow	1 day
MDS Screening Panel	2-3 days	CD34+ HSC analysis	4 hrs
B/T Minimal Residual Disease panel	2 days	Clonality Assay/	1 day
T Minimal Residual Disease panel	2 days	Customised panel for LL	2-3 days

 

4. Myeloma Lab: The lab is involved in the diagnosis and monitoring of multiple myeloma patients. A good number of patients suspected to have a plasma cell disorder are catered by the department. List of Services and turnaround time (TAT) are as follows:

Services	TAT	Services	TAT
Serum Protein Electrophoresis	3-4 days	Serum Immunofixation	3-4 days
Serum Free Light Chain	3-4 days	Beta 2 microglobulin	3-4 days
Serum/Plasma Viscosity	1-2 days	Urine Protein electrophoresis	3-4 days

 

5. Microbial Hematology Lab: This lab is created lab with an aim to perform the specialized investigations related to microbial infestation in pateitns of Stem cells transplant and other hematological disorders.

Services	TAT	Services	TAT
Glactomannan Antigen assay	4 days	-	-

 

6. Transplant biology lab: The transplant biology lab is involved in Human Leucocyte Antigen (HLA) typing for the potential bone marrow/haematopoietic stem cell transplantation patient.  Family screening is done using sequence specific primer based low to intermediate resolution HLA typing. Post-transplant monitoring of patients with chimerism analysis is also being performed using variable number tandem repeats (VNTR) sequences on a capillary sequencer. List of Services and turnaround time (TAT) are as follows:

Services	TAT	Services	TAT
HLA Typing (Class I & II), Low resolution	5 days	HLA Typing Class II	5 days
Chimerism analysis	4         days

 

7. Molecular Haematology lab: The molecular haematology lab is involved in the diagnosis and monitoring of hematological neoplasm. Several RNA and DNA based tests are performed in the lab. List of Services and turnaround time (TAT) are as follows:

Services	TAT	Services	TAT
BCR-ABL1 qualitative analysis	5 days	JAK2 V617F Mutation	5 days
BCR-ABL1 Quantitative assay- IS	7 days	CALR Mutation	5 days
RT- PCR  for AML-ETO	5 days	MPL Mutation	5 days
RT-PCR for PML-RARA	2 days	NPM1 mutation	5 days
RT PCR for Inv16	5 days	FLT3 mutation	5 days
RT-PCR for MLL-AF4	5 days	BCR-ABL1 TKD Mutation	7 days
RT-PCR for TEL-AML	5 days	B RAF V600E mutation	5 days
RT- PCR for E2A-PBX	5 days	MYD88 mutation	5 days
CMV DNA PCR	2   days

 

8.  Cancer Cytogenetics lab: The cancer cytogenetic lab is involved in conventional karyotyping and fluorescence in situ hybridization assay for the patients with haematological neoplasm. Additionally, chromosome breakage analysis using stress cytogenetics is also being performed.

Test Parameter	(TAT)	Test Parameter	(TAT)
ALL Reflex FISH panel	7 days	FISH for ABL1 rearrangement	7 days
Multiple Myeloma FISH panel	7-10 days	FISH for CRLF2 gene rearrangement	7 days
Double Hit Lymphoma FISH panel, c-MYC, BCL2 & BCL6 gene rearrangement	7 days	FISH for PDGFR genes rearrangement	7 days
FISH for t(15;17)/PML-RARa fusion	24 hours	FISH for PDGFRagene rearrangement	7 days
FISH for ATM(11q22)/TP53(17p13) gene deletion in CLL)	7 days	FISH for PDGFRbgene rearrangement	7 days
FISH for inv(16)/CBFb rearrangement	7 days	FISH for KMT2A (MLL) gene rearrangement	7 days
FISH for del(7q)/(5q)	7 days	FISH for t(12;21)/ETV6-RUNX1 fusion	7 days
FISH for c-MYC gene rearrangement	7 days	FISH for BCL2 (18q21) gene rearrangement	7 days
FISH for t(11;14)/IgH-CCND1 fusion	7 days	FISH for IgH(14q32) gene rearrangement	7 days
FISH for del(17p13)/TP53 deletion	7 days	FISH for t(9;22)/BCR-ABL1	7 days
Stress Cytogenetics (Chromosomal breakage study from Peripheral blood)	2-3wks	FISH for ABL1 gene rearrangement	7 days
Karyotyping from BM aspirate/ PB sample	14-21 days	FISH for CRLF2 gene rearrangement	7 days